Anti-BLBP抗体- Neuronal Marker (ab27171)

概述

  • 产品名称
    Anti-BLBP抗体- Neuronal Marker
    参阅全部 BLBP 一抗
  • 描述
    兔多克隆抗体to BLBP - Neuronal Marker
  • 经测试应用
    适用于: IHC-FoFr, IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: NFDEYMKALG VGFATRQVGN VTKPTVIISQ EGDKVVIRTL , corresponding to amino acids 16-65 of Human Fatty Acid Binding Protein 7

性能

应用

Our Abpromise guarantee covers the use of ab27171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/100.
ICC/IF 1/200. Fix cells with paraformaldehyde and block with BSA.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers.
  • 组织特异性
    Expressed in brain and other neural tissues.
  • 序列相似性
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B FABP antibody
    • B-FABP antibody
    • BFABP antibody
    • BLBP antibody
    • Brain lipid binding protein antibody
    • Brain lipid-binding protein antibody
    • Brain-type fatty acid-binding protein antibody
    • DKFZp547J2313 antibody
    • FABP 7 antibody
    • FABP7 antibody
    • FABP7_HUMAN antibody
    • FABPB antibody
    • Fatty Acid Binding Protein 7 antibody
    • Fatty acid binding protein 7 brain antibody
    • Fatty acid binding protein brain antibody
    • Fatty acid-binding protein 7 antibody
    • Fatty acid-binding protein, brain antibody
    • Mammary derived growth inhibitor related antibody
    • Mammary-derived growth inhibitor related antibody
    • MRG antibody
    • OTTHUMP00000017119 antibody
    see all

图片

  • ab27171 at 1/200 staining human foetal neural precursors by ICC/IF. The cells were paraformaldehyde fixed, blocked with BSA and incubated with the antibody for 12 hours. An Alexa Fluor ® 594 conjugated goat anti-rabbit IgG was used as the secondary.  Human neurons and astrocytes derived from neural precursors were used as the negative control. ab27171 has weak non-specific staining of the nucleolus however staining of the cytoplasm has not been detected in negative controls.

    See Abreview

  • Lane 1 : Anti-BLBP antibody - Neuronal Marker (ab27171) at 5 µg/ml
    Lane 2 : Anti-BLBP antibody - Neuronal Marker (ab27171) at 2.5 µg/ml
    Lane 3 : Anti-BLBP antibody - Neuronal Marker (ab27171) at 1.25 µg/ml

    Lane 1 : Lysate prepared from human fetal brain
    Lane 2 : Lysate prepared from human fetal brain
    Lane 3 : Lysate prepared from human fetal brain

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • Anti-BLBP antibody - Neuronal Marker (ab27171) at 1.25 µg/ml + Recombinant Human BLBP protein (ab83028) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

文献

This product has been referenced in:
  • Bagasrawala I  et al. N-Methyl D-Aspartate Receptor Antagonist Kynurenic Acid Affects Human Cortical Development. Front Neurosci 10:435 (2016). Read more (PubMed: 27746712) »
  • Hui SP  et al. Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish. PLoS One 10:e0143595 (2015). Read more (PubMed: 26630262) »

See all 13 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Nothobranchius furzeri Tissue sections (brain)
Permeabilization
Yes - Triton
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Formaldehyde
Username

Jolien Van houcke

Verified customer

提交于 Oct 13 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton x-100
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Tamar Licht

Verified customer

提交于 Jun 23 2017

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2012

Thank you for your response.

This is to let you know that I have placed a new order for you - for one vial ofab27171 as a free of charge replacement and this item has been added to Order No:1084016 .

I hope the second vial will work...

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Thank you for your response.

Currently, we only have stock in the US (3 vials) and the JP office (1 vial) but none in our UK office. These vials are from the same bleed and purification your customer had so I am unable to offer you a new batc...

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Thank you for contacting us again and for providing some further details.

It may well be that the antibody has been accidentally damaged during shipment or transfer. I would suggest aliquotting this product and storing at -20°C or -80°...

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Thank you for your enquiry regarding ab27171 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

Would you be so kind to let me know in w...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum,1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

提交于 Oct 05 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat embryo d16 spinal cord T/S)
Specification
Rat embryo d16 spinal cord T/S
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Oct 16 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (foetal neural precursors)
Specification
foetal neural precursors
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 Jan 10 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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