Bisulfite Conversion试剂盒-全细胞(ab117126)

概述

  • 产品名称Bisulfite Conversion试剂盒-全细胞
    参阅全部 DNA modification 试剂盒
  • 样品类型
    Saliva, Urine, Tissue, Adherent cells, Suspension cells
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Human
    预测可用于: A wide range of mammals
  • 产品概述

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

    Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.

     Bisulfite Modification Kit - Whole Cell (ab117126) allows the user to perform DNA methylation analysis and modify DNA directly from cells or tissues within only 3 hours. ab117126 is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384-well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.

    Modified DNA is eluted and suitable for real time MS-PCR as well as for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray.

    Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

  • 说明

     

     

     

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab117126 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration. Minimum starting material: 100-20000 cells / 1-100µg tissue / 0.2-2mm2 tissue section sample. Optimal starting material: 500-5000 cells / 5-20µg tissue / 0.5-1mm2 tissue section.

    Bisulfite Conversion Kit - Whole Cell 图像

    • MCF-7 cells were modified using ab117126. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of ß-actin was used.

    实验方案

    Bisulfite Conversion Kit - Whole Cell (ab117126)参考文献

    ab117126 has not yet been referenced specifically in any publications.

    Product Wall

    After bisulfite the DNA will be in ssDNA form and can not be directly used for ligation with illumina's adaptor. We recommend to use ab117127 (enhancer program) to bisulfite modifying DNA after ligation with Illumina's adaptor as indicated by Illumina ...

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    Thank you very for having patience!
    If use extracted DNA, the procedure can be processed starting from step 3 with slight modification:

    3. Add 1 ml of EBM5 to 1 vial of EBM4, followed by adding 60 µl of EBM6 to create the EBM4/EBM5/EBM6 so...

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    Thank you for contacting us.
    The kit can be used with tissue as low as 1 microgram and with paraffin embedded tissue sections. The procedure is given in attached booklet;
    http://www.abcam.com/ps/products/117/ab117126/documents/ab117126%20EpiS...

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    Thank you for your patience regarding this shipment of the digestion buffer for ab117126. I am very sorry for the delay. I have issued a free of charge replacement.You should receive this tomorrow.

    Please note that this free of charge replace...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"