Biotinylated山羊抗兔IgG (H+L) (Ready to use) (ab64256)
概述
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产品名称
Biotinylated山羊抗兔IgG (H+L) (Ready to use)
参阅全部 IgG 试剂 -
经测试应用
适用于: IHC-Pmore details -
常规说明
Staining protocol- Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
- Add appropriate hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
- Perform appropriate pretreatment if required. Wash 3 times in buffer.
- Apply appropriate protein blocking solution and incubate for 5 minutes at room temperature to block non specific background staining. Wash 1 time in buffer.
- Apply primary antibody and incubate according to manufacturer's protocol.
- Wash 4 times in buffer. Apply Ready to use Biotinylated Goat anti Rabbit IgG (ab64256) solution and incubate for 10-15 minutes at room temperature. Wash 4 times in buffer.
- Apply enzyme labelled streptavidin and incubate according to manufacturer’s protocol.
- Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
- Add enough drops of Haematoxylin to cover the section. Incubate for 1 minute.
- Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme labeled streptavidin, and substrate chromogen.
性能
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形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.0985% Sodium azide
Constituent: PBS -
Concentration information loading...
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Reagent说明
This reagent is intended to be used in a labeled streptavidin biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme labeled streptavidin, and substrate chromogen. -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
细胞定位
Secreted
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64256于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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说明 |
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IHC-P
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (101)
ab64256 被引用在 101 文献中.
- Pakkarato S et al. Discrete localization patterns of PIP5Kγ and PLCβ3 working sequentially in phosphoinositide-cycle within mouse sensory neuron somata. Microsc Res Tech 86:351-358 (2023). PubMed: 36579633
- Rämö JT et al. Genome-wide screen of otosclerosis in population biobanks: 27 loci and shared associations with skeletal structure. Nat Commun 14:157 (2023). PubMed: 36653343
- Ashraf UM et al. Intrarenal Dopaminergic System Is Dysregulated in SS-Resp18mutant Rats. Biomedicines 11:N/A (2023). PubMed: 36672619
- Zhang Y et al. Copper Induces Cognitive Impairment in Mice via Modulation of Cuproptosis and CREB Signaling. Nutrients 15:N/A (2023). PubMed: 36839332
- Becker V et al. Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha. Mol Ther Nucleic Acids 31:421-436 (2023). PubMed: 36845338