Anti-Biotin Ligase Epitope Tag抗体(ab106159)

概述

  • 产品名称Anti-Biotin Ligase Epitope Tag抗体
  • 描述
    兔多克隆抗体to Biotin Ligase Epitope Tag
  • 特异性ab106159 detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BirA). The antibody will recognize the BirA tag irrespective of whether the tag is biotinylated.
    Western blot analysis of Bacillus subtilis strains producing either BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ showed strong and specific reactivity to the antisera.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Other
  • 免疫原

    Synthetic peptide corresponding to Escherichia coli Biotin Ligase Epitope Tag.
    Sequence:

    GLNDIFEAQKIEWH

  • 阳性对照
    • Whole cell lysates from Bacillus subtilis producing BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ
  • 常规说明Dilute only prior to immediate use.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000.

Anti-Biotin Ligase Epitope Tag antibody (ab106159)参考文献

ab106159 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (Hek293T)
Gel Running Conditions Reduced Denaturing (4–15% Mini Protean TGX (Bio-Rad))
Loading amount 30 µg
Treatment Biotin 50 uM for 24 h
Specification Hek293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"