Anti-Biotin抗体(FITC) (ab53469)

概述

  • 产品名称Anti-Biotin抗体(FITC)
    参阅全部 Biotin 一抗
  • 描述
    兔多克隆抗体to Biotin (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性Reacts with free biotin and biotinylated proteins regardless of species.
  • 经测试应用适用于: ICC/IFmore details
  • 免疫原

    d-Biotin conjugated to KLH

  • 常规说明

    Fluorescein isothionate; Ab/FITC ratio=approximately 1/20.

性能

应用

Our Abpromise guarantee covers the use of ab53469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.

靶标

  • 相关性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 别名
    • Vitamin B4 antibody

Anti-Biotin antibody (FITC) 图像

  • ab53469 staining Biotin in human ovarian cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton ×100 in PBS and blocking with 5% serum for 30min at 230C was performed. Samples were incubated with primary antibody (1/100) for 1 hour at 23°C.

    See Abreview

Anti-Biotin antibody (FITC) (ab53469)参考文献

ab53469 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (ovarian)
Specification ovarian
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jun 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"