Anti-Biotin抗体[BN-34] (HRP) (ab49358)

概述

  • 产品名称Anti-Biotin抗体[BN-34] (HRP)
    参阅全部 Biotin 一抗
  • 描述
    小鼠单克隆抗体[BN-34] to Biotin (HRP)
  • 偶联物HRP
  • 特异性The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins
  • 经测试应用适用于: In situ hybridization, IHC-P, Indirect ELISA, Dot blot, FluroSpotmore details
  • 免疫原

    Biotin conjugated to KLH

  • 阳性对照
    • Human tonsils and human IgG
  • 常规说明

    The immunoglobulin G fraction of the ascites fluid is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.

性能

应用

Our Abpromise guarantee covers the use of ab49358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
In situ hybridization
IHC-P
Indirect ELISA
Dot blot
FluroSpot
  • 应用说明Dot: 1/4000.
    Fluorospot: 1/80000.
    IHC-P: 1/300.
    ISH: Use at an assay dependent dilution.
    I-ELISA: 1/30000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • 别名
      • Vitamin B4 antibody

    Anti-Biotin antibody [BN-34] (HRP) (ab49358)参考文献

    ab49358 has not yet been referenced specifically in any publications.

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