概述

  • 产品名称
  • 描述
    兔多克隆抗体to Biotin
  • 特异性
    Reacts with free biotin and biotinylated antibody and proteins
  • 经测试应用
    适用于: WB, Competitive ELISA, IP, ICC/IFmore details
  • 免疫原

    Full length protein conjugated to KLH

性能

应用

Our Abpromise guarantee covers the use of ab53494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
Competitive ELISA Use at an assay dependent dilution. (sensitivity: 0.01 ng/mL).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性
    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 别名
    • Vitamin B4 antibody

Anti-Biotin antibody (ab53494)参考文献

This product has been referenced in:
  • Na Pombejra S  et al. The Metalloprotease, Mpr1, Engages AnnexinA2 to Promote the Transcytosis of Fungal Cells across the Blood-Brain Barrier. Front Cell Infect Microbiol 7:296 (2017). Read more (PubMed: 28713781) »
  • Saredi G  et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature 534:714-718 (2016). WB . Read more (PubMed: 27338793) »

See all 9 Publications for this product

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (RWP1)
Specification
RWP1
Total protein in input
500 µg
Username

Abcam user community

Verified customer

提交于 Feb 14 2014

Thank you for contacting us.

The optimal dilutions or concentrations has to be determined by end user. We can recommend using the antibody at starting dilution 10ug/ml.

I hope this information is helpful to you. Please do not hesitate...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册