The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
1/200 - 1/2000.
1/1000 - 1/30000.
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
Use at an assay dependent concentration.
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Vitamin B4 antibody
Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John Andersen
Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary Streptavidin-HRP Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
This image is courtesy of an Abreview submitted by Mr John Andersen
Banuelos CA et al. Sintokamide A Is a Novel Antagonist of Androgen Receptor That Uniquely Binds Activation Function-1 in Its Amino-terminal Domain. J Biol Chem291:22231-22243 (2016).
Read more (PubMed: 27576691) »
Waaijers S et al. A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. BMC Biol14:66 (2016).
Read more (PubMed: 27506200) »