Anti-Biotin抗体[3E6] (ab36406)

概述

  • 产品名称Anti-Biotin抗体[3E6]
    参阅全部 Biotin 一抗
  • 描述
    小鼠单克隆抗体[3E6] to Biotin
  • 经测试应用适用于: WB, ELISAmore details
  • 免疫原

    Chemical/ Small Molecule - biotinylated denatured Ovalbumin.

  • 常规说明


    Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.

性能

应用

Our Abpromise guarantee covers the use of ab36406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms.
ELISA 1/20000. Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin.

靶标

  • 相关性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 别名
    • Vitamin B4 antibody

Anti-Biotin antibody [3E6] (ab36406)参考文献

ab36406 has not yet been referenced specifically in any publications.

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I will have to double-check with the lab regarding glycosylation of the antibodies since I do not have this information readily at hand. I will let you know once I have received th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (In Vitro Translation)
Loading amount 0.005 µg
Specification In Vitro Translation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 May 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"