概述

  • 产品名称Anti-Biotin抗体[39-15D9]
    参阅全部 Biotin 一抗
  • 描述
    小鼠单克隆抗体[39-15D9] to Biotin
  • 特异性This antibody reacts with both free biotin and biotin bound to a carrier protein. There is no cross reaction with the free carrier protein (determined by ELISA).
  • 经测试应用适用于: ELISA, WBmore details
  • 免疫原

    Full length protein conjugated to KLH.

性能

应用

Our Abpromise guarantee covers the use of ab3780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/5000.

靶标

  • 相关性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 别名
    • Vitamin B4 antibody

Anti-Biotin antibody [39-15D9] (ab3780)参考文献

This product has been referenced in:
  • Potluri LP  et al. ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division. J Bacteriol 194:5334-42 (2012). Read more (PubMed: 22843850) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This antibody does bind the d-isomer of biotin. Please do not hesitate to contact us if you have additional questions.

Thank you for your enquiry. The hybridoma cell line comes from the antibody's originator (the source's name is proprietary) and unfortunately we do not have any references at this time. If you have any additional questions, please contact us again.

Thank you for your enquiry. We are sorry to inform you that there is no concentration of the immunoglobulin for antibodies that are not affinity purified. Please take a look at the suggested dilutions for each application listed on the datasheet.

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On Western blot membran you can detect biotinylated proteins but not free biotine. Therefore the size of the bands depends of the molecular weight of the biotintylated protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"