概述

  • 产品名称Anti-BIN1抗体
    参阅全部 BIN1 一抗
  • 描述
    兔多克隆抗体to BIN1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BIN1.

  • 阳性对照
    • WB: Human skeletal muscle tissue lysate. ICC/IF: methanol fixed PC12 cells.

性能

应用

Our Abpromise guarantee covers the use of ab113544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60, 65 kDa (predicted molecular weight: 65 kDa).

靶标

  • 功能May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
  • 组织特异性Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
  • 疾病相关Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
  • 序列相似性Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Phosphorylated by protein kinase C.
  • 细胞定位Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMPH 2 antibody
    • AMPH2 antibody
    • Amphiphysin 2 antibody
    • Amphiphysin II antibody
    • Amphiphysin like protein antibody
    • amphiphysin-like antibody
    • Amphiphysin-like protein antibody
    • AMPHL antibody
    • Bin1 antibody
    • BIN1_HUMAN antibody
    • Box Dependant MYC Interacting Protein 1 antibody
    • Box-dependent myc-interacting protein 1 antibody
    • Bridging integrator 1 antibody
    • DKFZp547F068 antibody
    • MGC10367 antibody
    • MGC105358 antibody
    • Myc box dependent interacting protein 1 antibody
    • Myc box-dependent-interacting protein 1 antibody
    • SH3P9 antibody
    see all

Anti-BIN1 antibody 图像

  • Anti-BIN1 antibody (ab113544) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 49 kDa,60 kDa (possible isoform). We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab113544 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113544 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BIN1 antibody (ab113544)参考文献

ab113544 has not yet been referenced specifically in any publications.

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