Anti-Bim抗体, prediluted (ab15185)

概述

  • 产品名称
    Anti-Bim抗体, prediluted
    参阅全部 Bim 一抗
  • 描述
    兔多克隆抗体to Bim, prediluted
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide (Human) (N terminal).

  • 阳性对照
    • HeLa cells, Placenta

应用

Our Abpromise guarantee covers the use of ab15185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC-P: 1/1. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
    • 组织特异性
      Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
    • 序列相似性
      Belongs to the Bcl-2 family.
    • 结构域
      The BH3 motif is required for Bcl-2 binding and cytotoxicity.
    • 细胞定位
      Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BCL2 like 11 antibody
      • B2L11_HUMAN antibody
      • BAM antibody
      • Bcl 2 interacting protein Bim antibody
      • Bcl 2 related ovarian death agonist antibody
      • Bcl-2-like protein 11 antibody
      • BCL2 interacting mediator of cell death antibody
      • BCL2 like 11 (apoptosis facilitator) antibody
      • BCL2 like protein 11 antibody
      • Bcl2-interacting mediator of cell death antibody
      • Bcl2-L-11 antibody
      • Bcl2l11 antibody
      • BIM alpha6 antibody
      • BIM antibody
      • BIM beta6 antibody
      • BIM beta7 antibody
      • BimEL antibody
      • BimL antibody
      • BOD antibody
      see all

    文献

    ab15185 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Specification
    pancreas
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm citric
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册