The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at a concentration of 10 µg/ml. Use cells permeabilized with 0.03% saponin
ICC: Use at an assay dependent dilution. Use permeabilized cells
IP: Use 1 - 2µg/100-500 µg protein in 1 ml lysate.
WB: Use at a concentration of 1 - 2 µg/ml (reduced conditions). Predicted molecular weight: 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Belongs to the Bcl-2 family.
The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.