概述

  • 产品名称Anti-Bim抗体
    参阅全部 Bim 一抗
  • 描述
    兔多克隆抗体to Bim
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) (N terminal).

  • 阳性对照
    • HeLa cells. Placenta

应用

Our Abpromise guarantee covers the use of ab15184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P 1/25. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • 组织特异性Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • 细胞定位Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Anti-Bim antibody 图像

  • ab15184, at 1:25 dilution, staining Bim in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
  • All lanes : Anti-Bim antibody (ab15184)

    Lane 1 : Total cell protein extracts prepared from CEMC7-14 cells, un-treated.
    Lane 2 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 2 hours.
    Lane 3 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 6 hours.
    Lane 4 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 24 hours.

    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa (possible isoform),16 kDa (possible isoform).

    Image from Dr JT Lynch et al, Mol Cancer. 2010 Feb 15;9:38, Fig 5.

Anti-Bim antibody (ab15184)参考文献

This product has been referenced in:
  • Lynch JT  et al. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer 9:38 (2010). WB ; Human . Read more (PubMed: 20156337) »
  • Bouchard C  et al. FoxO transcription factors suppress Myc-driven lymphomagenesis via direct activation of Arf. Genes Dev 21:2775-87 (2007). Read more (PubMed: 17974917) »

See all 2 Publications for this product

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