Anti-Bile salt-activated lipase抗体(ab87431)

概述

  • 产品名称Anti-Bile salt-activated lipase抗体
    参阅全部 Bile salt-activated lipase 一抗
  • 描述
    兔多克隆抗体to Bile salt-activated lipase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Xenopus laevis, Drosophila melanogaster, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Bile salt-activated lipase.

    (Peptide available as ab87432.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Pancreas; Mouse Pancreas

性能

应用

Our Abpromise guarantee covers the use of ab87431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性Defects in Bile salt activated lipase (CEL) are a cause of maturity onset diabetes of the young type 8 with exocrine dysfunction (MODY8) [MIM:609812], also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY [MIM:606391] is an autosomal dominant form of diabetes mellitus. The pancreas serves both endocrine and exocrine functions. The endocrine cells are found in the islets of Langerhans. They synthesize insulin and other hormones, and are involved in the pathogenesis of diabetes mellitus. The exocrine cells produce bicarbonate and digestive enzymes and are involved in the pathogenesis of pancreatic malabsorption. The localization of the islets within exocrine pancreatic tissue is suggestive of an interdependency and cross talk between these two cell populations in their normal and in their abnormal function.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • BAL antibody
    • Bile salt-stimulated lipase antibody
    • BSDL antibody
    • BSSL antibody
    • Bucelipase antibody
    • Carboxyl ester lipase (bile salt stimulated lipase) antibody
    • Carboxyl ester lipase antibody
    • CEase antibody
    • CEL antibody
    • CELL antibody
    • Cholesterol esterase antibody
    • FAP antibody
    • FAPP antibody
    • Fetoacinar pancreatic protein antibody
    • LIPA antibody
    • Lysophospholipase, pancreatic antibody
    • MODY8 antibody
    • Pancreatic lysophospholipase antibody
    • Sterol esterase antibody
    see all

Anti-Bile salt-activated lipase antibody 图像

  • All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa (possible cleavage fragment).

    Exposure time : 30 seconds
  • All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 74,100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa (possible cleavage fragment).

    Exposure time : 30 seconds
  • IHC image of Bile salt-activated lipase staining in mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87431, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Bile salt-activated lipase antibody (ab87431)参考文献

ab87431 has not yet been referenced specifically in any publications.

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