Anti-Bile Acid Receptor NR1H4抗体(ab85606)


  • 产品名称
    Anti-Bile Acid Receptor NR1H4抗体
    参阅全部 Bile Acid Receptor NR1H4 一抗
  • 描述
    兔多克隆抗体to Bile Acid Receptor NR1H4
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Hamster, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 Bile Acid Receptor NR1H4.

    (Peptide available as ab97929.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HepG2 Whole Cell; Human Kidney Tissue; Human Small Intestine.



Our Abpromise guarantee covers the use of ab85606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).


  • 功能
    Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAR antibody
    • Bile acid receptor antibody
    • Farnesoid X activated receptor antibody
    • Farnesoid X receptor antibody
    • Farnesoid X-activated receptor antibody
    • Farnesol receptor HRR 1 antibody
    • Farnesol receptor HRR-1 antibody
    • Farnesol receptor HRR1 antibody
    • FXR antibody
    • HRR 1 antibody
    • HRR1 antibody
    • NR1H4 antibody
    • NR1H4_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 4 antibody
    • Retinoid X receptor interacting protein 14 antibody
    • Retinoid X receptor-interacting protein 14 antibody
    • RIP 14 antibody
    • RIP14 antibody
    • RXR interacting protein 14 antibody
    • RXR-interacting protein 14 antibody
    see all


  • All lanes : Anti-Bile Acid Receptor NR1H4 antibody (ab85606) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes
  • ICC/IF image of ab85606 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85606, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and HepG2 cells at 5µg/ml.


ab85606 has not yet been referenced specifically in any publications.


There are currently no Customer reviews or Questions for ab85606.
Please use the links above to contact us or submit feedback about this product.