Anti-Biglycan抗体(ab94460)

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概述

  • 产品名称
    Anti-Biglycan抗体
    参阅全部 Biglycan 一抗
  • 描述
    兔多克隆抗体to Biglycan
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SEAKLTGIPK DLPETLNELH LDHNKIQAIE LEDLLRYSKL YRLGLGHNQI) of Human Biglycan (NP_001702).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab94460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May be involved in collagen fiber assembly.
  • 组织特异性
    Found in several connective tissues, especially in articular cartilages.
  • 序列相似性
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻译后修饰
    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SEMDX antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all

Anti-Biglycan antibody 图像

  • Western blot - Biglycan antibody (ab94460)
    Western blot - Biglycan antibody (ab94460)
    Anti-Biglycan antibody (ab94460) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa

实验方案

Anti-Biglycan antibody (ab94460)参考文献

ab94460 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab94460？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Western blot abreview for Anti-Biglycan antibody

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Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Tissue lysate - whole (Aorta)
Specification
Aorta
Treatment
Non deglycosylated - Deglycosylated (PNGaseF)
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Feb 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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