概述

  • 产品名称Anti-Biglycan抗体
    参阅全部 Biglycan 一抗
  • 描述
    小鼠单克隆抗体to Biglycan
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-369 of Human Biglycan

性能

应用

Our Abpromise guarantee covers the use of ab54855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 1 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20801503

靶标

  • 功能May be involved in collagen fiber assembly.
  • 组织特异性Found in several connective tissues, especially in articular cartilages.
  • 序列相似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻译后修饰The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all

Anti-Biglycan antibody 图像



  • Predicted band size : 42 kDa
    Biglycan antibody (ab54855) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ab54855 (2µg/ml) staining biglycan in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1/ in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing A431 cells stained with ab54855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54855, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Biglycan antibody (ab54855)参考文献

This product has been referenced in:
  • Dumont N  et al. Breast fibroblasts modulate early dissemination, tumorigenesis, and metastasis through alteration of extracellular matrix characteristics. Neoplasia 15:249-62 (2013). WB ; Human . Read more (PubMed: 23479504) »
  • Li Y  et al. Age-dependent alterations of decorin glycosaminoglycans in human skin. Sci Rep 3:2422 (2013). Read more (PubMed: 23939413) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - other (Mesenchymal stem cell)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Mesenchymal stem cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Apr 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"