概述

  • 产品名称
    Anti-Biglycan抗体
    参阅全部 Biglycan 一抗
  • 描述
    兔多克隆抗体to Biglycan
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Biglycan.

    (Peptide available as ab69790.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: Human Skin Tissue HepG2 Whole Cell Jurkat Whole Cell THP1 Whole Cell Rat Spleen Tissue Rat Thymus Tissue Mouse Kidney Tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta.

性能

应用

Our Abpromise guarantee covers the use of ab49701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    May be involved in collagen fiber assembly.
  • 组织特异性
    Found in several connective tissues, especially in articular cartilages.
  • 序列相似性
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻译后修饰
    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SEMDX antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all

图片

  • All lanes : Anti-Biglycan antibody (ab49701) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : THP1 whole cell lysate (ab7913)
    Lane 5 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)
    Lane 6 : Thymus (Rat) Tissue Lysate
    Lane 7 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 26 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of Biglycan staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49701, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab49701 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49701, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Mayer C  et al. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells. Sci Rep 6:37128 (2016). IHC-P ; Human . Read more (PubMed: 27849015) »
  • Nadanaka S  et al. Roles of EXTL2, a member of the EXT family of tumour suppressors, in liver injury and regeneration processes. Biochem J 454:133-45 (2013). Read more (PubMed: 23734945) »

See all 7 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (nasal septum)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 0,3 % Collagenase & 0,5 U/mL Chondroitinase ABC
Permeabilization
No
Specification
nasal septum
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Ms. Katharina Tluczynski

Verified customer

提交于 Apr 25 2016

Application
Western blot
Sample
Pig Cell lysate - whole cell (Porcine Vocal Fold Fibroblasts (VFF))
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
1 µg
Specification
Porcine Vocal Fold Fibroblasts (VFF)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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