重组
RabMAb

Anti-Bid抗体[Y8] (ab32060)

概述

  • 产品名称
    Anti-Bid抗体[Y8]
    参阅全部 Bid 一抗
  • 描述
    兔单克隆抗体[Y8] to Bid
  • 特异性
    This antibody does not cross-react with other Bcl-2 family members.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues before the BH3 domain of human Bid.

  • 阳性对照
    • Jurkat cell lysate and human prostate carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Bid antibody (HRP) [Y8] (ab201754)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab32060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10.

靶标

  • 功能
    The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
  • 组织特异性
    Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
  • 结构域
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻译后修饰
    TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
  • 细胞定位
    Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptic death agonist antibody
    • Apoptotic death agonist BID antibody
    • BH3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist p11 antibody
    • BH3 interacting domain death agonist p13 antibody
    • BH3 interacting domain death agonist p15 antibody
    • BH3-interacting domain death agonist p11 antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • BID_HUMAN antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p11 BID antibody
    • p13 BID antibody
    • p15 BID antibody
    • p22 BID antibody
    see all

Anti-Bid antibody [Y8] 图像

  • Anti-Bid antibody [Y8] (ab32060) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32060 at 1/100 dilution.
  • All lanes : Anti-Bid antibody [Y8] (ab32060) at 1/500 dilution

    Lane 1 : Whole cell lysate prepared from breast cancer clinical extract
    Lane 2 : Whole cell lysate prepared from breast cancer clinical extract
    Lane 3 : Whole cell lysate prepared from MCF7 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit IgG-HRP at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 22 kDa


    Exposure time : 25 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab32060 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32060, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Bid antibody [Y8] (ab32060)参考文献

This product has been referenced in:
  • Wang C  et al. Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors. BMC Cancer 16:545 (2016). WB ; Human . Read more (PubMed: 27464624) »
  • Stefanowicz-Hajduk J  et al. Pennogenyl Saponins from Paris quadrifolia L. Induce Extrinsic and Intrinsic Pathway of Apoptosis in Human Cervical Cancer HeLa Cells. PLoS One 10:e0135993 (2015). WB ; Human . Read more (PubMed: 26295969) »

See all 10 Publications for this product

Product Wall

Thank you for your reply. In terms of rabbit antibodies to Bid that have been tested in ICC, we have only ab32060 http://www.abcam.com/Bid-antibody-Y8-ab32060.html It has not been tested in mouse yet, though. The immunogen is a synthetic peptide corres...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast Cancer)
Loading amount
20 µg
Specification
Breast Cancer
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Oral Cancer)
Loading amount
20 µg
Specification
Oral Cancer
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 21 2011

Application
Flow Cytometry
Sample
Human Cell (U937)
Specification
U937
Fixation
Leucoperm Reagent A
Permeabilization
Yes - Leucoperm Reagent B
Gating Strategy
Live Cells
Username

Abcam user community

Verified customer

提交于 Sep 09 2008

Application
Western blot
Sample
Human Cell lysate - whole cell (U937 and PBMC)
Loading amount
4e+006 cells
Specification
U937 and PBMC
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
2% Milk with 1% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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