概述

  • 产品名称Anti-Bid抗体
    参阅全部 Bid 一抗
  • 描述
    兔多克隆抗体to Bid
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human Bid. The epitope is found in isoforms 1 and 2 (but not isoform 3) of human BID.

  • 阳性对照
    • HeLa cells.
  • 常规说明Centrifuge tube to remove product from lid.

性能

应用

Our Abpromise guarantee covers the use of ab14436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.

靶标

  • 功能The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
  • 组织特异性Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
  • 结构域Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻译后修饰TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
  • 细胞定位Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptic death agonist antibody
    • Apoptotic death agonist BID antibody
    • BH3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist p11 antibody
    • BH3 interacting domain death agonist p13 antibody
    • BH3 interacting domain death agonist p15 antibody
    • BH3-interacting domain death agonist p11 antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • BID_HUMAN antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p11 BID antibody
    • p13 BID antibody
    • p15 BID antibody
    • p22 BID antibody
    see all

Anti-Bid antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Bid with ab14436 at 1/1000 (1µg/ml). Detection: DAB.
  • Anti-Bid antibody (ab14436) at 1 µg/ml + Hela whole cell lysate at 40 µg

    Predicted band size : 29 kDa
    ab14436 at 1µg/ml staining human BID in HeLa cell lysate by Western blot (ECL).

Anti-Bid antibody (ab14436)参考文献

ab14436 has not yet been referenced specifically in any publications.

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