功能May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
序列相似性Belongs to the BicD family.
结构域The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
翻译后修饰Phosphorylated by NEK9 in vitro.
细胞定位Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.
Detection of Human BICD2 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab117818 at 6 µg/mg of lysate. ab117818 at 0.4 µg/ml was used for subsequent WB detection of the immunoprecipitate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-BICD2 antibody (ab117818)参考文献
has not yet been referenced specifically in any publications.