Anti-BICD2抗体(ab117818)
Key features and details
- Rabbit polyclonal to BICD2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BICD2抗体
参阅全部 BICD2 一抗 -
描述
兔多克隆抗体to BICD2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region within amino acids 375-425 of Human BICD2 (NP_056065.1).
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阳性对照
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab117818 was affinity purified using an epitope specific to BICD2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117818于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the BicD family. -
结构域
The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2. -
翻译后修饰
Phosphorylated by NEK9 in vitro. -
细胞定位
Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology. - Information by UniProt
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数据库链接
- Entrez Gene: 23299 Human
- Omim: 609797 Human
- SwissProt: Q8TD16 Human
- Unigene: 436939 Human
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别名
- bA526D8.1 antibody
- Bic D 2 antibody
- Bic-D 2 antibody
see all
图片
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All lanes : Anti-BICD2 antibody (ab117818) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes -
Detection of Human BICD2 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab117818 at 6 µg/mg of lysate. ab117818 at 0.4 µg/ml was used for subsequent WB detection of the immunoprecipitate.
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab117818 被引用在 7 文献中.
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270
- Kumari A et al. Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions. J Cell Biol 220:N/A (2021). PubMed: 34709360
- Spriggs CC et al. Golgi-associated BICD adaptors couple ER membrane penetration and disassembly of a viral cargo. J Cell Biol 219:N/A (2020). PubMed: 32259203
- Tsai MH et al. Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation. Acta Neuropathol Commun 8:106 (2020). PubMed: 32665036
- Saeki E et al. Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor. PLoS One 13:e0209296 (2018). PubMed: 30557316
- Redwine WB et al. The human cytoplasmic dynein interactome reveals novel activators of motility. Elife 6:N/A (2017). PubMed: 28718761
- Dharan A et al. Bicaudal D2 facilitates the cytoplasmic trafficking and nuclear import of HIV-1 genomes during infection. Proc Natl Acad Sci U S A 114:E10707-E10716 (2017). PubMed: 29180435