概述

  • 产品名称
  • 描述
    兔多克隆抗体to BHMT
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within the internal sequence amino acids 199-393 of Human BHMT.

  • 阳性对照
    • HepG2 cell lysate SG xenograft (IHC)

性能

应用

Our Abpromise guarantee covers the use of ab96415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能
    Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  • 组织特异性
    Found exclusively in liver and kidney.
  • 通路
    Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
    Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
  • 序列相似性
    Contains 1 Hcy-binding domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Betaine homocysteine methyltransferase antibody
    • Betaine homocysteine S methyltransferase 1 antibody
    • Betaine homocysteine S methyltransferase antibody
    • Betaine--homocysteine S-methyltransferase 1 antibody
    • BHMT antibody
    • BHMT1 antibody
    • BHMT1_HUMAN antibody
    • EC 2.1.1.5 antibody
    • Epididymis secretory sperm binding protein Li 61p antibody
    • HEL S 61p antibody
    see all

图片

  • Anti-BHMT antibody (ab96415) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 45 kDa
    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab96415 at 1:100 dilution.
  • All lanes : Anti-BHMT antibody (ab96415) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 45 kDa

文献

This product has been referenced in:
  • Carter WG  et al. Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury. Biochem Biophys Res Commun 458:626-31 (2015). Read more (PubMed: 25684186) »
  • Zhang Y  et al. Homocysteine homeostasis and betaine-homocysteine S-methyltransferase expression in the brain of hibernating bats. PLoS One 8:e85632 (2013). WB, IHC-P ; Bat . Read more (PubMed: 24376891) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - other (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Wayne Carter

Verified customer

提交于 Mar 28 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - other (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Wayne Carter

Verified customer

提交于 Mar 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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