The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
组织特异性Found exclusively in liver and kidney.
通路Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.