概述

  • 产品名称Anti-BHC80 / PHF21A抗体
    参阅全部 BHC80 / PHF21A 一抗
  • 描述
    兔多克隆抗体to BHC80 / PHF21A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human BHC80/ PHF21A.

    (Peptide available as ab41630)

  • 阳性对照
    • ab41631 gave a positive result in the following Tissue Lysates Brain (Human) Brain (Mouse) Brain (Rat)

性能

应用

Our Abpromise guarantee covers the use of ab41631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa).

靶标

  • 功能Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.
  • 组织特异性Highly expressed in brain. Expressed at much lower level in other tissues.
  • 序列相似性Contains 1 A.T hook DNA-binding domain.
    Contains 1 PHD-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BHC80a antibody
    • BM-006 antibody
    • BRAF35-HDAC complex protein BHC80 antibody
    • BRAF35/HDAC2 complex (80 kDa) antibody
    • KIAA1696 antibody
    • PF21A_HUMAN antibody
    • PHD finger protein 21A antibody
    • PHF21A antibody
    see all

Anti-BHC80 / PHF21A antibody 图像

  • All lanes : Anti-BHC80 / PHF21A antibody (ab41631) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Brain (Rat) Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

Anti-BHC80 / PHF21A antibody (ab41631)参考文献

This product has been referenced in:
  • Lin S  et al. Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. J Biol Chem 288:6238-47 (2013). Read more (PubMed: 23349461) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (brain sections)
Specification brain sections
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"