Anti-beta V Tubulin抗体- Carboxyterminal end (ab82366)

概述

  • 产品名称
    Anti-beta V Tubulin抗体- Carboxyterminal end
    参阅全部 beta V Tubulin 一抗
  • 描述
    兔多克隆抗体to beta V Tubulin - Carboxyterminal end
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human beta V Tubulin (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P07437

  • 阳性对照
    • A2058 cytoplasmic lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/1000.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 序列相似性
    Belongs to the tubulin family.
  • 结构域
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • M40 antibody
    • TBB5_HUMAN antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 5 chain antibody
    • Tubulin beta antibody
    • Tubulin beta chain antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

图片

  • Anti-beta V Tubulin antibody - Carboxyterminal end (ab82366) at 1/50 dilution + A2058 cell line lysates at 35 µg

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

实验方案

文献

ab82366 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab82366.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册