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Purified rat brain tubulin.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.
Our Abpromise guarantee covers the use of ab11309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100. This dilution was determined by direct immunofluorescent labeling of cultured chicken fibroblasts or BHK cells. The conjugate may be used in double labeling experiments with fluorescein tagged antibodies.|
ab11309 staining beta Tubulin in Mouse cultured cortical neurons by Immunocytochemistry/Immunofluorescence. Cells were plated on a poly-L-Lysine coated cover slip before being washed twice with PBS and fixed with 4% PFA at room temperature. Cells were permeabilized in 0.5%(w/v) saponin in PBS prior to blocking in 10% fetal calf serum in PBS-saponin for 2 hours at 23°C. The primary antibody was diluted 1/1000 in the blocking buffer and incubated with the sample for 2 hours at 23°C.
After additional washing cover splips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI (Invitrogen). The image was acquired with a 40x objective.
This image is courtesy of an anonymous Abreview.Lysis Buffer was 1% Triton X-100, 20mM TrisHCl pH 7.5, 10mM EGTA, 150mM NaCl, protease inhibitors. The image was acquired by scanning the blot with a FLA-9000 Starion laserscanner at 300V and 200µm resolution. The above mentioned exposure time is just the time it took to scan the blot but does not correlate to signal intensity.
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