Anti-beta Tubulin抗体[TUB 2.1] (Cy3 ®) (ab11309)

概述

  • 产品名称
    Anti-beta Tubulin抗体[TUB 2.1] (Cy3 ®)
    参阅全部 beta Tubulin 一抗
  • 描述
    小鼠单克隆抗体[TUB 2.1] to beta Tubulin (Cy3 ®)
  • 偶联物
    Cy3 ®. Ex: 552nm, Em: 565nm
  • 特异性
    It reacts with the chymotryptic beta-Lc and beta-Sc tubulin fragments and does not seem to exhibit mouse brain beta-tubulin isoform-related specificity
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Hamster, Cow, Human
  • 免疫原

    Purified rat brain tubulin.

  • 表位
    Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446).
  • 阳性对照
    • Cultured chicken fibroblasts or BHK cells.
  • 常规说明

    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

    The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.

性能

应用

Our Abpromise guarantee covers the use of ab11309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.
ICC/IF 1/100. This dilution was determined by direct immunofluorescent labeling of cultured chicken fibroblasts or BHK cells. The conjugate may be used in double labeling experiments with fluorescein tagged antibodies.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 疾病相关
    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • 序列相似性
    Belongs to the tubulin family.
  • 结构域
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • 细胞定位
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

图片

  • ab11309 staining beta Tubulin in Mouse cultured cortical neurons by Immunocytochemistry/Immunofluorescence. Cells were plated on a poly-L-Lysine coated cover slip before being washed twice with PBS and fixed with 4% PFA at room temperature. Cells were permeabilized in 0.5%(w/v) saponin in PBS prior to blocking in 10% fetal calf serum in PBS-saponin for 2 hours at 23°C. The primary antibody was diluted 1/1000 in the blocking buffer and incubated with the sample for 2 hours at 23°C.
    After additional washing cover splips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI (Invitrogen). The image was acquired with a 40x objective.

    See Abreview

  • All lanes : Anti-beta Tubulin antibody [TUB 2.1] (Cy3 ®) (ab11309) at 1/1000 dilution

    Lane 1 : HEK cell lysate at 5 µg
    Lane 2 : HEK cell lysate at 40 µg


    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview.

    Lysis Buffer was 1% Triton X-100, 20mM TrisHCl pH 7.5, 10mM EGTA, 150mM NaCl, protease inhibitors. The image was acquired by scanning the blot with a FLA-9000 Starion laserscanner at 300V and 200µm resolution. The above mentioned exposure time is just the time it took to scan the blot but does not correlate to signal intensity.

    See Abreview

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary chondrocytes)
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
Primary chondrocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Mr. Masato Nomura

Verified customer

提交于 Sep 29 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,5% (w/v) saponin in PBS
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 27 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mixed glia prepared from mouse brain)
Loading amount
5 µg
Specification
mixed glia prepared from mouse brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 human embryonic kidney)
Loading amount
5 µg
Specification
293 human embryonic kidney
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured cortical neurons)
Specification
cultured cortical neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,5%(w/v) saponin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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