Anti-beta Tubulin抗体[TU-12] (ab24385)


  • 产品名称Anti-beta Tubulin抗体[TU-12]
    参阅全部 beta Tubulin 一抗
  • 描述
    小鼠单克隆抗体[TU-12] to beta Tubulin
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    The epitope recognised by this antibody is highly conserved and therefore the antibody cross reacts with a wide range of species.
  • 免疫原

    Full length native protein (purified) of brain microtubule protein MTP-1 (Pig)

  • 表位The antibody reacts with an epitope located within amino acids 345-430 of C-terminal domain of beta-tubulin subunit.
  • 阳性对照
    • porcine brain lysate



Our Abpromise guarantee covers the use of ab24385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ICC: Use at an assay dependent dilution. Ab46814 is suitable for ICC/IF as it is the same clone directly fluorescent labelled.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 38 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • 组织特异性Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
    • 疾病相关Cortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • 序列相似性Belongs to the tubulin family.
    • 结构域The highly acidic C-terminal region may bind cations such as calcium.
    • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
    • 细胞定位Cytoplasm, cytoskeleton.
    • Information by UniProt
    • 别名
      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    Anti-beta Tubulin antibody [TU-12] (ab24385)参考文献

    ab24385 has not yet been referenced specifically in any publications.

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