Anti-beta Tubulin抗体, prediluted (ab15569)

概述

  • 产品名称Anti-beta Tubulin抗体, prediluted
    参阅全部 beta Tubulin 一抗
  • 描述
    兔多克隆抗体to beta Tubulin, prediluted
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Gerbil, Sea Urchin, Fungi
  • 免疫原

    Synthetic peptide:

    NDLVSEYQQYQDATA

    , corresponding to amino acids 416-430 of Human beta Tubulin.

  • 阳性对照
    • MAD109 cells, Skin and lung

性能

应用

Our Abpromise guarantee covers the use of ab15569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: Working dilution, ready to use for 10 minutes at RT.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • 组织特异性Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
    • 疾病相关Cortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • 序列相似性Belongs to the tubulin family.
    • 结构域The highly acidic C-terminal region may bind cations such as calcium.
    • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
    • 细胞定位Cytoplasm, cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    Anti-beta Tubulin antibody, prediluted 图像

    • ab15569 staining beta Tubulin in human lung by Immunohistochemistry (FFPE-sections).

    Anti-beta Tubulin antibody, prediluted (ab15569)参考文献

    ab15569 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Valve interstitial cells and valve endothelial cel)
    Gel Running Conditions Reduced Non-Denaturing (Native)
    Loading amount 25 µg
    Specification Valve interstitial cells and valve endothelial cel
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C
    Username

    Dr. hessah alshammari

    Verified customer

    提交于 Feb 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"