概述

  • 产品名称
    Anti-beta Tubulin抗体
    参阅全部 beta Tubulin 一抗
  • 描述
    山羊多克隆抗体to beta Tubulin
  • 特异性
    ab21057 detects a band of ~50kDa in Hela and A431 lysates (data for A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, African green monkey
    预测可用于: Rat, Chicken
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human beta Tubulin.

    (Peptide available as ab20775.)

  • 阳性对照
    • HeLa whole cell lysate, HeLa nuclear lysate and A431 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab21057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta Tubulin peptide (ab20775).
ICC/IF 1/1000.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 疾病相关
    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • 序列相似性
    Belongs to the tubulin family.
  • 结构域
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • 细胞定位
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody 图像

  • All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti Goat polyclonal at 1/10000 dilution

    Predicted band size : 50 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)

    ab21057 detects a band of ~ 50kDa in Hela whole cell lysate, HeLa nuclear lysate and A431 lysates (data for HeLa nuclear and A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.

     

     

  •    Ab21057 staining beta-tubulin in asynchronous HeLa cells (green). Cells were counterstained with DAPI (red) to show the nucleus. Secondary antibody: Donkey anti-Goat antibody conjugated to Alexafluor 488 ® (Please refer to the Abreview for further details).

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 150 seconds

    Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
    Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
    20ug of Lysate per lane and detection using ab21057 diluted to 1ug/ml.
    Lane 1: Hela cell lysate.
    Lane 2: Jurkat cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HEK293 cell lysate
    Lane 5: HepG2 cell lysate.

  • ab21057 staining beta tubulin in CV1 (fibroblasts) cells from African green monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and permeabilized with methanol. Samples were incubated with primary antibody (1/500 in PBS + 5% BSA) for 1 hour 30 minutes at 22°C. An Alexa Fluor® 488-conjugated Donkey anti-goat polyclonal was used as the secondary antibody (1/200).

    See Abreview

Anti-beta Tubulin antibody (ab21057)参考文献

This product has been referenced in:
  • Xu S  et al. ANRIL lncRNA triggers efficient therapeutic efficacy by reprogramming the aberrant INK4-hub in melanoma. Cancer Lett N/A:N/A (2016). WB ; Human . Read more (PubMed: 27461581) »
  • Anand U  et al. Mechanisms underlying clinical efficacy of Angiotensin II type 2 receptor (AT2R) antagonist EMA401 in neuropathic pain: clinical tissue and in vitro studies. Mol Pain 11:38 (2015). ICC/IF . Read more (PubMed: 26111701) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Aug 08 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (platelet)
Permeabilization
Yes - 0.2% Triton X100
Specification
platelet
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
platelet
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart whole cell lysate)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
30 µg
Treatment
Doxycyline to induce transgene
Specification
Heart whole cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

提交于 Nov 18 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 30 2015

I have issued a free of charge replacement.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV1 (fibroblasts))
Specification
CV1 (fibroblasts)
Fixative
Methanol
Permeabilization
Yes - Methanol
Username

Mr. Nir Drayman

Verified customer

提交于 Aug 16 2012

Laut SwissProt wird dieses Protein ubiquitär expremiert, daher auch im Gehirn. Er wird Ihnen daher wohl nicht nur Neuronen sondern auch die Gliazellen anfärben. Der Antikörper wurde bisher in der ICC aber nicht in der IHC getestet. Wir haben verschi...

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Frau Z befindet sich in ihrem wohlverdienten Urlaub und ist leider erst im Juli wieder zu erreichen.
In der Zwischenzeit werde ich mich gerne um Ihren Fall kümmern.
Vielen Dank für Ihre Antwort und für diese weiteren Informationen.
Es tu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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