概述

  • 产品名称
    Anti-beta Tubulin抗体
    参阅全部 beta Tubulin 一抗
  • 描述
    兔多克隆抗体to beta Tubulin
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Cow, Human, Rice
    预测可用于: Rat, Chicken, Guinea pig, Dog, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Xenopus tropicalis
  • 免疫原

    Synthetic peptide:

    NDLVSEYQQYQDATA

    , corresponding to amino acids 416-430 of Human beta Tubulin.

  • 阳性对照
    • MAD109 cells, Skin and lung, HeLa cell line

性能

应用

Our Abpromise guarantee covers the use of ab15568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 19509307

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/200. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 疾病相关
    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • 序列相似性
    Belongs to the tubulin family.
  • 结构域
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • 细胞定位
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody 图像

  • All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).

    See Abreview

  • ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa


    Exposure time : 30 seconds

Anti-beta Tubulin antibody (ab15568)参考文献

This product has been referenced in:
  • Heim JB  et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A 114:3933-3938 (2017). WB ; Mouse, Human . Read more (PubMed: 28348210) »
  • Zollo M  et al. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain 140:940-952 (2017). WB, IF . Read more (PubMed: 28334956) »

See all 30 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate pH6
Sample
Sea Urchin Tissue sections (Testes)
Specification
Testes
Permeabilization
No
Fixative
Formaldehyde
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Abcam user community

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提交于 Oct 06 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (IMR32, Human Neuroblatoma)
Specification
IMR32, Human Neuroblatoma
Permeabilization
Yes - 0.3% triton X 100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 26 2013

Thank you for contacting us and your interest in our products.
We would advise trying the antibody at a dilution of 1 ug/mL for both flow cytometry and ICC staining. This is only an initial recommendation, you may need to increase or decrease this...

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Thank you for your reply.

I have returned the Abreview to you for revision.

The notes section should be at the bottom third of the form.

Please let me know if you have any questions.

Thank you for contacting us. I am very glad to hear that you have taken advantage of our 100% Abreview program.

I have found the review which you have submitted and wish to thank you for this. In order to take advantage of the review code,...

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Thank you for your response. We have predicted cross reactivity with this species, but we have not yet tested in Saccharomyces cerevisiae and did not know what results would occur when using this in yeast. While products used in 'predicted to react'...

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Thank you for that information.

I have just a few more questions regarding this. What species are you testing this product against? Do you run these westerns natively or in a reduced and denatured condition?

If we cannot remedy th...

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Application
Western blot
Sample
Neurospora crassa Cell lysate - whole cell (non)
Loading amount
35 µg
Specification
non
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 21 2011

Application
Western blot
Sample
Sclerotinia sclerotiorum Cell lysate - whole cell (non)
Loading amount
35 µg
Specification
non
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 13 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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