Anti-beta Tubulin抗体[37B] (ab101019)

概述

  • 产品名称Anti-beta Tubulin抗体[37B]
    参阅全部 beta Tubulin 一抗
  • 描述
    小鼠单克隆抗体[37B] to beta Tubulin
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein fragment: GEGMDEMEFT EAESNMNDLV SEYQQYQDAT AEEEEDFGEE AEEEA , corresponding to amino acids 400-444 of Human beta Tubulin (NP_821133.1).

  • 阳性对照
    • WB: SW480 whole cell lysate ICC/IF: HeLa cell

性能

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应用

Our Abpromise guarantee covers the use of ab101019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/10000.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 疾病相关Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • 序列相似性Belongs to the tubulin family.
  • 结构域The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • 细胞定位Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody [37B] 图像

  • Anti-beta Tubulin antibody [37B] (ab101019) at 1/5000 dilution + SW480 whole cell lysate

    Predicted band size : 50 kDa
  • ab101019, at 1/10 000 dilution, staining beta Tubulin in HeLa cells by Immunofluorescence.

Anti-beta Tubulin antibody [37B] (ab101019)参考文献

ab101019 has not yet been referenced specifically in any publications.

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