Anti-beta Sarcoglycan抗体(ab83699)

概述

  • 产品名称
    Anti-beta Sarcoglycan抗体
    参阅全部 beta Sarcoglycan 一抗
  • 描述
    兔多克隆抗体to beta Sarcoglycan
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (

    FSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGN

    ) of Human beta Sarcoglycan (NP_000223)

  • 阳性对照
    • Human fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab83699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 功能
    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • 组织特异性
    Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.
  • 疾病相关
    Limb-girdle muscular dystrophy 2E
  • 序列相似性
    Belongs to the sarcoglycan beta/delta/gamma/zeta family.
  • 翻译后修饰
    Disulfide bonds are present.
  • 细胞定位
    Cell membrane, sarcolemma. Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 43 kDa dystrophin associated glycoprotein antibody
    • 43 kDa dystrophin-associated glycoprotein antibody
    • 43DAG antibody
    • A3b antibody
    • Beta SG antibody
    • Beta-sarcoglycan antibody
    • Beta-SG antibody
    • dystrophin-associated glycoprotein, 43-kD antibody
    • LGMD2E antibody
    • Limb girdle muscular dystrophy 2E (non linked families) antibody
    • Sarcoglycan beta antibody
    • sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) antibody
    • SGC antibody
    • SGCB antibody
    • SGCB_HUMAN antibody
    see all

图片

  • Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)



    Gel concentration: 12%

实验方案

文献

ab83699 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (Cardiac muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE Novex 10% Bis-Tris Protein Gels)
Loading amount
10 µg
Specification
Cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Federica Montanaro

Verified customer

提交于 Apr 13 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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