兔多克隆抗体to beta Sarcoglycan
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (
) of Human beta Sarcoglycan (NP_000223)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.
Limb-girdle muscular dystrophy 2E
Belongs to the sarcoglycan beta/delta/gamma/zeta family.
Disulfide bonds are present.
Cell membrane, sarcolemma. Cytoplasm, cytoskeleton.
Information by UniProt
- 43 kDa dystrophin associated glycoprotein antibody
- 43 kDa dystrophin-associated glycoprotein antibody
- 43DAG antibody
Western blot - Anti-beta Sarcoglycan antibody (ab83699)
Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
35 kDaObserved band size:
40 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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