Anti-beta III Tubulin抗体[TU-20] (FITC) (ab25770)

概述

• 产品名称
  Anti-beta III Tubulin抗体[TU-20] (FITC)
  参阅全部 beta III Tubulin 一抗
• 描述
  小鼠单克隆抗体[TU-20] to beta III Tubulin (FITC)
• 偶联物
  FITC. Ex: 493nm, Em: 528nm
• 特异性
  This antibody recognizes the peptide sequence ESESQGPK (amino acids 441-448) of human class III beta tubulin specific for neurones.
• 经测试应用
  适用于: ICC, IHC-FoFrmore details
• 种属反应性
  All (the recognized epitope is conserved within all species).
• 免疫原

  Synthetic peptide: corresponding to amino acids (C)441-448 (

  ESESQGPK

  ) of beta III Tubulin conjugated to maleimide activated KLH via cysteine added to the N terminus of the neurone specific peptide.

  Run BLAST with Run BLAST with
• 常规说明

  The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at +4°C.
• 存储溶液
  Preservative: 15mM Sodium Azide.
  Constituents: PBS, pH 7.4.
• Concentration information loading...
• 纯度
  >95% by SDS-PAGE
• 纯化说明
  Size exclusion chromatography.
• 克隆
  单克隆
• 克隆编号
  TU-20
• 同种型
  IgG1
• 研究领域
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • Tubulin

相关产品

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

应用

靶标

• 功能
  Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
• 组织特异性
  Expression is primarily restricted to central and peripheral nervous system.
• 疾病相关
  Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
• 序列相似性
  Belongs to the tubulin family.
• 结构域
  The highly acidic C-terminal region may bind cations such as calcium.
• 翻译后修饰
  Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
• 细胞定位
  Cytoplasm > cytoskeleton.

• Target information above from: UniProt accession Q13509 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 别名
  • beta 3 tubulin antibody
  • beta-4 antibody
  • CDCBM antibody

  • CDCBM1 antibody
  • CFEOM3 antibody
  • CFEOM3A antibody
  • FEOM3 antibody
  • M(beta)3 antibody
  • M(beta)6 antibody
  • MC1R antibody
  • Neuron specific beta III Tubulin antibody
  • Neuron-specific class III beta-tubulin antibody
  • QccE-11995 antibody
  • QccE-15186 antibody
  • TBB3_HUMAN antibody
  • Tubb 3 antibody
  • TUBB3 antibody
  • TUBB4 antibody
  • Tubulin beta 3 antibody
  • Tubulin beta 3 chain antibody
  • Tubulin beta 4 antibody
  • Tubulin beta III antibody
  • Tubulin beta-3 chain antibody
  • Tubulin beta-4 chain antibody
  • Tubulin beta-III antibody

  see all

实验方案

• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols