FITC荧光Anti-beta III Tubulin抗体[TU-20] (ab25770)
Key features and details
- FITC Mouse monoclonal [TU-20] to beta III Tubulin
- Suitable for: ICC, IHC-Fr
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
FITC荧光Anti-beta III Tubulin抗体[TU-20]
参阅全部 beta III Tubulin 一抗 -
描述
FITC荧光小鼠单克隆抗体[TU-20] to beta III Tubulin -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
This antibody recognizes the peptide sequence ESESQGPK (amino acids 441-448) of human class III beta tubulin specific for neurones. -
经测试应用
适用于: ICC, IHC-Frmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human beta III Tubulin aa 400-500 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: Q13509 -
阳性对照
- ICC: Neuro2a cell line
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常规说明
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading...
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纯度
Size exclusion -
纯化说明
Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
TU-20 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25770于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 1 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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ICC
Use a concentration of 1 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
组织特异性
Expression is primarily restricted to central and peripheral nervous system. -
疾病相关
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
序列相似性
Belongs to the tubulin family. -
结构域
The highly acidic C-terminal region may bind cations such as calcium. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 22152 Mouse
- SwissProt: Q9ERD7 Mouse
- Unigene: 40068 Mouse
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别名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab25770 被引用在 7 文献中.
- Zhang S et al. Alteration of long non-coding RNAs and mRNAs expression profiles by compound heterozygous ASXL3 mutations in the mouse brain. Bioengineered 12:6935-6951 (2021). PubMed: 34559584
- Rharass T et al. Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/ß-catenin/ROS signaling dependent manner. J Biomed Sci 24:78 (2017). PubMed: 29037191
- Rharass T et al. Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/ß-catenin pathway activation to facilitate cell differentiation. J Biol Chem 289:27937-51 (2014). ICC ; Human . PubMed: 25124032
- Gilmont RR et al. Bioengineering of physiologically functional intrinsically innervated human internal anal sphincter constructs. Tissue Eng Part A 20:1603-11 (2014). PubMed: 24328537
- Lemcke H et al. Neuronal differentiation requires a biphasic modulation of gap junctional intercellular communication caused by dynamic changes of connexin43 expression. Eur J Neurosci 38:2218-28 (2013). PubMed: 23607708
- Raghavan S et al. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141:310-9 (2011). IHC-Fr ; Mouse . PubMed: 21463628
- Launay S et al. HtrA1-dependent proteolysis of TGF-beta controls both neuronal maturation and developmental survival. Cell Death Differ 15:1408-16 (2008). IHC-FoFr ; Mouse . PubMed: 18551132