Anti-beta II Tubulin抗体[6B1] (ab28036)

概述

  • 产品名称Anti-beta II Tubulin抗体[6B1]
    参阅全部 beta II Tubulin 一抗
  • 描述
    小鼠单克隆抗体[6B1] to beta II Tubulin
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, all Mammals
  • 免疫原

    Synthetic peptide, corresponding to amino acids CEEEEGEDEA at the C terminus of human beta II Tubulin.

性能

应用

Our Abpromise guarantee covers the use of ab28036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the tubulin family.
  • 结构域The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA506K6.1 antibody
    • Beta2 antibody
    • class II beta tubulin isotype antibody
    • class IIa beta-tubulin antibody
    • class IIb beta-tubulin antibody
    • Class IVb beta tubulin antibody
    • dJ40E16.7 antibody
    • DKFZp566F223 antibody
    • FLJ98847 antibody
    • M(beta)2 antibody
    • MGC8685 antibody
    • OTTHUMP00000015956 antibody
    • OTTHUMP00000015964 antibody
    • TBB4B_HUMAN antibody
    • TUBB 2 antibody
    • TUBB 2A antibody
    • TUBB 2C antibody
    • TUBB antibody
    • TUBB PARALOG antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB2B antibody
    • TUBB2C antibody
    • Tubb4b antibody
    • Tubulin beta 2 antibody
    • Tubulin beta 2 chain antibody
    • Tubulin beta 2A antibody
    • Tubulin beta 2A chain antibody
    • Tubulin beta 2B antibody
    • Tubulin beta 2B chain antibody
    • Tubulin beta 2C antibody
    • Tubulin beta polypeptide antibody
    • Tubulin beta polypeptide 2 antibody
    • Tubulin beta polypeptide paralog antibody
    • Tubulin beta-2 chain antibody
    • Tubulin beta-2C chain antibody
    • Tubulin beta-4B chain antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta 2B class IIb antibody
    • tubulin, beta 4B class IVb antibody
    • Tubulin, beta, class IVB antibody
    • Tubulin, beta-4B antibody
    see all

Anti-beta II Tubulin antibody [6B1] 图像

  • Ab28036, at a dilution of 1/1000, staining beta II Tubulin (green) in methanol fixed HeLa cells by Immunofluoresence.
  • Ab28036, at a dilution of 1/1000, staining beta II Tubulin (green) in methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence.

Anti-beta II Tubulin antibody [6B1] (ab28036)参考文献

This product has been referenced in:
  • Kuznetsov AV  et al. H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochim Biophys Acta 1853:276-84 (2015). WB, IF ; Rat . Read more (PubMed: 25450968) »
  • Yancey DM  et al. Cardiomyocyte mitochondrial oxidative stress and cytoskeletal breakdown in the heart with a primary volume overload. Am J Physiol Heart Circ Physiol 308:H651-63 (2015). IHC-P ; Rat . Read more (PubMed: 25599572) »

See all 4 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Rat Tissue lysate - whole (gastroc)
Specification gastroc
Total protein in input 800 µg
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提交于 Feb 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"