概述

  • 产品名称Anti-beta I Tubulin抗体
    参阅全部 beta I Tubulin 一抗
  • 描述
    兔多克隆抗体to beta I Tubulin
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 174 - 438 of beta I Tubulin.

  • 阳性对照
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab96008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 疾病相关Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810484G07Rik antibody
    • Beta tubulin 1, class VI antibody
    • Class VI beta tubulin antibody
    • dJ543J19.4 antibody
    • M(beta)1 antibody
    • TBB1_HUMAN antibody
    • TUBB1 antibody
    • Tubulin beta 1 class VI antibody
    • Tubulin beta-1 chain antibody
    • Tubulin, beta 1 antibody
    • tubulin, beta1 antibody
    see all

Anti-beta I Tubulin antibody 图像

  • All lanes : Anti-beta I Tubulin antibody (ab96008) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96008 at 1/200 dilution.
    Bottom panel: merged with DNA probe.

Anti-beta I Tubulin antibody (ab96008)参考文献

This product has been referenced in:
  • Wang H  et al. Multiple mechanisms underlying acquired resistance to taxanes in selected docetaxel-resistant MCF-7 breast cancer cells. BMC Cancer 14:37 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24447372) »

See 1 Publication for this product

Product Wall

Thank you for sending the details.

It is clear that tag is a problem which is interfering with the antibody. It may be that due to stearic hindrance epitope is not fully exposed because of conformational changes in the target protein.

Read More

Thank you for contacting us.

I am sorry we have not tested any of anti tubilin I antibodies in GFP-tubulin 1 tag so we will not be able to recommend any specific antibody. Surely, the heavy tag like GFP affects the binding of antibody to prote...

Read More

I will forward these details on to our Distributor’s Team who would be happy to organise the free of charge replacement. Your customer may be interested to know that if needs be, double staining can be performed with primary antibodies from the sam...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital infor...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"