beta Hydroxybutyrate (beta HB) Assay试剂盒(Colorimetric) (ab83390)

概述

  • 产品名称
    beta Hydroxybutyrate (beta HB) Assay试剂盒(Colorimetric)
    参阅全部 Beta Hydroxybutyrate 试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Quantitative
  • 灵敏度
    > 0.01 mM
  • 范围
    0.01 mM - 0.2 mM
  • 检测时间
    0h 40m
  • 产品概述

    Abcam's beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390) utilizes beta HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 µL samples or 0.01-0.2 mM of beta HB samples.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Beta hydroxybutyrate in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Beta hydroxybutyrate measured in biological fluids showing quantity (nmol) per microliter of tested sample

  • beta HB Standard Curve: Assays were performed following ab83390 kit protocol.
  • Human serum samples were filtered using a 10 kda MW cut-off spin filter (ab93349) spun at 14,000 RPM for 30 minutes. Beta Hydrobutyrate (BHB) was detected on a 96-well plate using ab83390.

    Young control group (6 men, 25±1 years old).
    Age-matched control group (13 men, 69±7 years old)
    PD = Individuals diagnosed with idiopathic PD (15 men, 71±8 years old, duration of disease = 10±6 years, H&Y = 2.3±0.7, median = 2).

    A trend approaching significance in increased levels of BHB in the plasma of PD patients compared to age-matched controls was observed. BHB is known to fluctuate in a variety of conditions including starvation, diabetes mellitus and other co-morbidities.

  • The plasma levels of total BHB was detected using ab83390. Five rats were in each group. Samples were collected from tail vein blood and were transferred into EDTA-containing tubes. After centrifugation at 1500×g for 15 min at room temperature, the plasma was transferred into a new tube and stored at -80°C until use. Values are presented as the mean ± S.E. (**p<0.01).

实验方案

文献

This product has been referenced in:
  • Lourbopoulos A  et al. Inadequate food and water intake determine mortality following stroke in mice. J Cereb Blood Flow Metab 37:2084-2097 (2017). Read more (PubMed: 27449604) »
  • Zhao M  et al. Ketogenic diet improves the spatial memory impairment caused by exposure to hypobaric hypoxia through increased acetylation of histones in rats. PLoS One 12:e0174477 (2017). Read more (PubMed: 28355243) »

See all 6 Publications for this product

客户评价及客户问答

The detection agent does not quantify the oxobutyrate but the byproduct of the reaction by the dehydrogenase. The reaction byproducts are produced proportionally based on the amount of beta hydroxybutyrate in a specific sample. This is why this assay k...

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Thank you for your enquiry and interest in our products.

I can confirm that this kit (ab83390: beta Hydroxybutyrate (beta HB) Assay Kit) measuresonly the β-Hydroxybutyrate in the samples.

Hope this information has been helpful ...

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Thank you for your phone call a couple of weeks ago, and I sincerely apologize for the delay getting back to you.

I've forwarded your data to our assay expert in the lab, and the kit has been standardized using mammalian samples so it may req...

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For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filte...

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Thank you for confirming the product number.

Could you confirm if you are planning on making plasma/serum from the blood samples? If so, yes you can definitely use this kit with plasma and serum. The colour of heme and the red blood cells wo...

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The principle you have correct, but the samples need not have any diaphorase. Everything required for this assay to work is being provided in the kit components.

I hope this information has been useful for you. Please let me know if you have...

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Thank you for your inquiry.
I can confirm that frozen serum or plasma samples are suitable for this kit.
We did not experimentally test frozen versus fresh samples but would expect a slight loss of activity in frozen samples.
This will n...

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Thank you for your enquiry.

I can confirm that tissue samples in the form of homogonate can be used with this kit.

In this case, carefully cut up the tissue to small pieces, then lyse using the kit Assay Buffer. To remove interfer...

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Thank you for contacting us.

You can certainly use the spin column from XXXXXX however the procedure should be followed as per manufacturer's datasheet.

Regarding the high readings, this assay has not been standardized for drosophil...

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Puede contactarnos directamente en español, telefónicamente (91 114 65 54), o bien por correo electrónico, a:

mailto:orders@abcam.com Para tramitar pedidos y otras consultas relacionada...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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