Anti-beta Glucuronidase (GUS)抗体- Aminoterminal end (ab50148)

概述

  • 产品名称Anti-beta Glucuronidase (GUS)抗体- Aminoterminal end
    参阅全部 Beta Glucuronidase (GUS) 一抗
  • 描述
    兔多克隆抗体to beta Glucuronidase (GUS) - Aminoterminal end
  • 特异性ab50148 recognizes bacterial beta Glucuronidase expressed in transgenic tobacco plants.
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MLRPVETPTREIKKLD

  • 阳性对照
    • Cell extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing E. coli GUS.

性能

应用

Our Abpromise guarantee covers the use of ab50148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.
IHC-Fr Use at an assay dependent concentration. PubMed: 21825109

靶标

  • 相关性Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
  • 细胞定位Cytoplasmic
  • 数据库链接
    • 别名
      • Beta D glucuronidase antibody
      • Beta glucuronidase antibody
      • gurA antibody
      • UidA antibody

    Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)参考文献

    This product has been referenced in:
    • Cai Q  et al. Jasmonic acid regulates spikelet development in rice. Nat Commun 5:3476 (2014). WB . Read more (PubMed: 24647160) »
    • Jasik J  et al. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiol 157:563-73 (2011). IHC-Fr . Read more (PubMed: 21825109) »

    See all 2 Publications for this product

    Product Wall

    Thank you very much for your question. Indeed the ab50148 is a polylconal antibody of rabbit origin. The antibody has been purified by affinity to the immunogen and the isotype has not been determined. However the rabbit immune system is only capable o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"