beta Galactosidase Detection试剂盒(ab102534)

概述

  • 产品名称beta Galactosidase Detection试剂盒
    参阅全部 beta galactosidase 试剂盒
  • 测试
    250 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 产品概述

    The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ.

    Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase.

    Visit our FAQs page for tips and troubleshooting.

  • 说明

    This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (ab65351).

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity.
  • 细胞定位Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes.
  • 别名
    • Acid beta galactosidase
    • Beta galactosidase 1
    • EBP
    • ELNR1
    • Galactosidase beta 1
    • GLB1
    • MPS4B
    see all

应用

Our Abpromise guarantee covers the use of ab102534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

beta Galactosidase Detection Kit 图像

  • Gamma-radiation induced senescence in hFOB cells. The number of senescence-associated-β-galactosidase positive cells (left panel) cleary increases 72h after 8 Gy γ irradiation. Representative data was from one of a total of 3 indepent experiments.

    Image obtained from Li XH et al; PLOS One; 2012;7(5):e36604

实验方案

beta Galactosidase Detection Kit (ab102534)参考文献

This product has been referenced in:
  • Li XH  et al. REDD1 Protects Osteoblast Cells from Gamma Radiation-Induced Premature Senescence. PLoS One 7:e36604 (2012). Human . Read more (PubMed: 22629318) »

See 1 Publication for this product

Product Wall

The staining procedure will remain the same for suspension cells except the cells would have to be in a micro-centrifuge tube while the washing (add PBS, spin down, remove buffer) and incubation steps are performed. Once done, the cells can be aliquote...

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The protocol calls for fixing tissues for a short period of time (10 - 15 min at room temperature). Stored samples may not have enough enzymatic activity to do this assay. Residual enzymatic activity also depends on how high the formalin concentration ...

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You can keep both reagents at 37°C until the precipitate dissolves.

Thank you for your inquiry.

I can confirm that we have optimized ab102534 for cells.

We believe it is possible to use this kit with frozen tissues, I am sorry to confirm that we do not know what optimizations will be necessary.
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Thank you for contacting us.

I have obtained the following information from the lab:

1) ab102534 cannot be used for senescence assay.
ab65351 is used to detect senescence-associated expression of β-galactosidase (SA-&beta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"