Anti-beta Galactosidase抗体[DC1 4C7] (ab116)

概述

  • 产品名称Anti-beta Galactosidase抗体[DC1 4C7]
    参阅全部 beta Galactosidase 一抗
  • 描述
    小鼠单克隆抗体[DC1 4C7] to beta Galactosidase
  • 经测试应用适用于: IHC-P, IHC-Fr, WB, ELISAmore details
  • 免疫原

    beta Galactosidase EC.3.2.1.23 from E.coli.

  • 常规说明

    This monoclonal is also known as clone HD4C7 or HD 447C.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Phosphate buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆单克隆
  • 克隆编号DC1 4C7
  • 骨髓瘤P3-NS1/1-Ag4-1
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 23272238
IHC-Fr Use at an assay dependent concentration. PubMed: 20431063
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 细胞定位Cytoplasmic
  • 别名
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

Anti-beta Galactosidase antibody [DC1 4C7] 图像

  • ab116 staining beta Galactosidase in Human primary HGG aggregate sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer for 40 minutes. Samples were incubated with primary antibody (1/1000) for 1 hour at room temperature. A HRP-conjugated Rabbit anti-mouse IgG was used as the secondary antibody.

  • ab116 at a 1/500 dilution staining beta Galactosidase in murine stomach tissue by Immunohistochemistry. The secondary used was an anti-mouse IgG antibody at a dilution of 1/2000.

Anti-beta Galactosidase antibody [DC1 4C7] (ab116)参考文献

This product has been referenced in:
  • Smith SJ  et al. Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition. PLoS One 7:e52335 (2012). IHC-P . Read more (PubMed: 23272238) »
  • Nassiri M  et al. Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice. Arterioscler Thromb Vasc Biol 30:1423-9 (2010). IHC-Fr . Read more (PubMed: 20431063) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We do not have a recommended dilution of this antibody. The dilution employed will depend on the level of expression of your LacZ construct. H...

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Thank you for your enquiry and for submitting your technical questionnaire. I am sorry to hear that your customer has been having difficulties with this antibody. I have read their technical enquiry and they have tried some of the recommendations th...

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Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We would like to confirm that this antibody has not been tested for immunohistochemi...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Yes, ab616 has successfully been tested for application in IHC-formalin fixed paraffin embedded sections. We have had some feedback from customers who have used ab616 in IHC-P to detect beta gal in transgenic mice - you may read their comments by click...

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Thank you for your email. Neither ab116 or ab1047 have been tested yet for application in IHC. You may want to look at ab616 which has been tested in IHC. If you decide to try ab116 or ab1047 in IHC, please let us know how you get on and in return w...

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This anitbody has not yet been tested in immunocytochemistry

Yes this antibody does work in this application (although there maybe some post-translational modification of the b-gal fusion protein which may decrease the binding of the antibody)

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"