Anti-beta Galactosidase抗体[BGAL01] (ab3291)

概述

  • 产品名称Anti-beta Galactosidase抗体[BGAL01]
    参阅全部 beta Galactosidase 一抗
  • 描述
    小鼠单克隆抗体[BGAL01] to beta Galactosidase
  • 经测试应用适用于: IP, WBmore details
  • 免疫原

    Full length protein (E. coli).

  • 阳性对照
    • Purified recombinant / beta Galactosidase protein or transfected cell lines expressing beta Galactosidase tagged proteins.
  • 常规说明Molecular weight of beta Galactosidase = ~ 116 kDa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号BGAL01
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
  • 应用说明Immunoprecipitation (with protein G): use at a concentration of 2µg / mg protein lysate.
    Western Blotting: use at a concentration of 1µg / ml for 2hrs at RT.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    This antibody may be used for detection of beta Galactosidase expressed by the E.coli lacZ geneencoded in many cloned gene sequences, and servesas an indicator for fusion proteins encoded by aninserted DNA sequence.
  • 靶标

    • 相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • 细胞定位Cytoplasmic
    • 别名
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Anti-beta Galactosidase antibody [BGAL01] (ab3291)参考文献

    ab3291 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"