Anti-beta Galactosidase抗体[BG-02] (ab1047)

概述

  • 产品名称Anti-beta Galactosidase抗体[BG-02]
    参阅全部 beta Galactosidase 一抗
  • 描述
    小鼠单克隆抗体[BG-02] to beta Galactosidase
  • 经测试应用适用于: WB, ICC, ICC/IFmore details
  • 免疫原

    Full length native protein (purified) (E. coli).

  • 表位Recognizes a non-catalytic centre epitope of Beta-galactosidase from E. coli.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by precipitation methods.
  • 克隆单克隆
  • 克隆编号BG-02
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/50 for 18 hrs on mouse mammary cells (see Abreview for further information).

靶标

  • 相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 细胞定位Cytoplasmic
  • 别名
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

Anti-beta Galactosidase antibody [BG-02] 图像

  • Anti-beta Galactosidase antibody [BG-02] (ab1047) at 1 µg/ml + Purified E. coli beta-galactosidase

    Secondary
    Goat anti-mouse IgG (H+L)-HRP conjugate

    Performed under non-reducing conditions.

Anti-beta Galactosidase antibody [BG-02] (ab1047)参考文献

This product has been referenced in:

See all 7 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Mouse Tissue (Retina)
Specification Retina
Username

Abcam user community

Verified customer

提交于 Aug 13 2015



We also do not have electronic access to the publication

Folia Biol (Praha). 1992;38(6):350-7.

A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of reco...

Read More

The Mouse monoclonal [BG-02] to beta Galactosidase, ab1047, should be able to detect the beta Galactosidase with the same sequence as that expressed in E. coli. This should work whether the protein is overexpressed or not. It should also work with beta...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

Read More

If Chicken secondary is a problem then I would suggest choosing other beta galactosidase antibodies from the catalogue, with other host e.g. rabbit as ab4761. I hope this will help.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (whole brain)
Loading amount 50 µg
Specification whole brain
Treatment Samples from wild-type (control) mice and gene-trap mice expressing b-geo fusion proteins
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. Teresa Gunn

Verified customer

提交于 Jul 31 2007

Thank you for your enquiry. This antibody shows no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beta-galactosidase fusion proteins expressed in lambda expression vectors. It was successfull...

Read More

Thank you for your enquiry. I am sorry I can not answer the question; however, I did find the following information in a product specific publication for this clone by Slavickova et al., PubMed ID 1284241. In this publication I have found these ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary)
Specification Mammary
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Apr 05 2006

Thank you for your enquiry. To our knowledge, our beta Galactosidase antibody [BG-02]has yet to be tested by immunohistochemistry or on guinea pig tissue. All tested applications are specified on Abcam product datasheets. Should you require fur...

Read More

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"