Anti-beta Galactosidase抗体(ab9361)

概述

  • 产品名称
    Anti-beta Galactosidase抗体
    参阅全部 beta Galactosidase 一抗
  • 描述
    鸡多克隆抗体to beta Galactosidase
  • 宿主
    Chicken
  • 经测试应用
    适用于: ELISA, IHC-FrFl, ICC, IHC-FoFr, Flow Cyt, ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Full length native protein (purified). The immunogen was purified beta-galactosidase from Escherichia coli.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    0.01M PBS pH7.4, 15mM Na azide
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase.
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/1000 - 1/2000.
Flow Cyt Use at an assay dependent concentration.

ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 116 kDa.
IHC-P 1/200 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 相关性
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
    • 别名
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    图片

    • P0-adult mice were euthanized and perfused with 4% paraformaldehyde in PBS (PF). Their spinal cords were then post-fixed for 30–60 mins in 4% PF at 4°C (P0) or at room temperature (adult). Spinal cords were rinsed and cryoprotected in 20% sucrose in PBS (4°C) prior to embedding in OCT (Tissue-Tek). Immunostaining of frozen spinal sections was performed by incubating 20 µm thick sections with primary antibodies, which were then detected using species-specific secondary antibodies conjugated with Cy2, Cy3 and Cy5 or FITC. ab9361 was used at 1:1000.

    • ab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).

      See Abreview

    • Ab9361 staining Beta galactosidase in Mouse thyroid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed 10% buffered formalin, cut into 3-4 micron slices, blocked with 10% normal goat serum and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with the following primary antibodies; anti-BrdU, anti-Sca1 (ab109211, 1∶600), anti-NKX2-1 and anti-β-gal (ab9361, 1∶1000). Sections were incubated with the first primary antibody (anti-Sca1) for 1 hour at room temperature. After washing with PBS, sections were incubated with the first secondary antibody (Alexa Fluor 555 goat anti-rabbit IgG) and washed with PBS. Sections were then incubated with normal serum (5% rabbit serum) from the same host species as the first primary antibodies for 1 hour at room temperature and washed with PBS. Sections were further incubated with an excess of unconjugated Fab antibody derived from the same host species as the primary antibody for 1 hour at room temperature and washed with PBS. The sections were finally incubated with the mixed second primary antibodies (anti-BrdU, anti-β-gal, anti-NKX2-1) overnight at 4°C, washed with PBS, and were incubated with the second secondary antibody (Alexa Fluor 488 goat anti-rat IgG, Dylight 650 goat anti-chicken IgY, Dylight 594 goat anti-rabbit IgG) for 1 hour at room temperature and washed with PBS. DAPI dye was used to stain the nuclei of cells.

    • ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.

      See Abreview

    • ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.

      See Abreview

    文献

    This product has been referenced in:
    • Kim YJ  et al. WDR11-mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome. EMBO Rep 19:269-289 (2018). WB ; Mouse . Read more (PubMed: 29263200) »
    • Ansen-Wilson LJ  et al. Common basis for orofacial clefting and cortical interneuronopathy. Transl Psychiatry 8:8 (2018). IHC ; Mouse . Read more (PubMed: 29317601) »

    See all 276 Publications for this product

    客户评价及客户问答


    ab9361 was raised against E. coli beta galactosidase, which only has 11% homology with mouse beta galactosidase. In general, we like to see greater than 85% homology before we expect an antibody to cross react with an untested species. Thus, ab936...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. O...

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    Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie besprochen, habe ich eine kostenlose Ersatzlieferung des ab97135 für Sie in Auftrag gegeben. Sie hat die Referenznummer 1212...

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    Es tut mir Leid, dass sie Proble...

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    Thank you for contacting Abcam.

    Under our Abpromise we guarantee that the antibody ab9361 will work on mouse sections that have been frozen or paraffin embedded before sectioning. If the antibody fails to perform as we guarantee then we will ...

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    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for...

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    Thank you for your recent telephone enquiry.

    I can confirm that ab9361 Anti-beta Galactosidase antibodyis isolated via affinity chromatography from Chicken egg yolks and is then tested by IEP and ELISA afterwards. The ELISA performed is an i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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