Anti-beta Galactosidase抗体(ab4761)

概述

  • 产品名称Anti-beta Galactosidase抗体
    参阅全部 beta Galactosidase 一抗
  • 描述
    兔多克隆抗体to beta Galactosidase
  • 特异性Detects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.
  • 经测试应用适用于: IHC-P, WBmore details
  • 免疫原

    Full length native protein (purified) (E. coli).

  • 阳性对照
    • This antibody detects beta galactosidase in HeLa cells in WB

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 17268532
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).

靶标

  • 相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 细胞定位Cytoplasmic
  • 别名
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

Anti-beta Galactosidase antibody 图像

  • All lanes : Anti-beta Galactosidase antibody (ab4761) at 1/1000 dilution

    Lane 1 : Beta Gal recombinant protein at 0.1 µg
    Lane 2 : 20ug HeLa WCL + 0.1ug Beta Gal recombinant protein at 20 µg

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 116 kDa
    Observed band size : 116 kDa

Anti-beta Galactosidase antibody (ab4761)参考文献

This product has been referenced in:
  • Purmessur D  et al. A role for TNFa in intervertebral disc degeneration: a non-recoverable catabolic shift. Biochem Biophys Res Commun 433:151-6 (2013). Read more (PubMed: 23438440) »
  • Giagtzoglou N  et al. Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition. J Cell Sci 126:3686-96 (2013). Read more (PubMed: 23788431) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (colon)
Specification colon
Fixative formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH=6
Permeabilization No
Blocking step 2%BSA, 10% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

提交于 Jan 10 2013

Code de remise : xxxxxx
Date d’expiration : xxxxxxxx
Valeur: €xxxxxx

Je suis ravie que vous souhaitiez participer a notre offre et tester ab4761 chez la souris. Le code de réduction est xxxxxxxxxx. Ce code de r&ea...

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Merci de nous avoir contactés.

L’anticorps ab4761 a été testé en IHC-P et WB. L’espèce testée en IHC-P est l’humain.

Voir la publication suivante : Hachiya A et al. Gene t...

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Merci de nous avoir contactés.

Nous n'avons pas encore testé l’anticorps ab4761 chez la souris. Cependant, puisque nous n'avons pour l'instant pas d'image IHC-P chez la souris à mettre sur la fiche technique du ab476...

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Thank you for your inquiry.
I can confirm that ab4761 will detect beta Galactosidase from E.coli origin.
If beta Galactosidase is overexpressed (ectopically expressed) in human tissue than this antibody is suitable for this.
We tested th...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

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Thank you for your reply.

It is absolutely correct that you will get the full price of ab4761 as a discount off a future order.

If you would like to take part in this program, please let me know. I will be happy to issue a testing d...

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Thank you very much for your interest in ab4761 and ab616.

I confirm that we cannot guarantee ab616 for IHC and we also do not recommend this antibody for this application.

To our knowledge, ab4761 has not been tested in IHC - Who...

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Thank you for your inquiry.
I am happy to confirm that ab4761 is tested and guaranteed for IHC-P.
The following publication even used this antibody with human/ viral transfected samples:
Hachiya A et al. Gene transfer in human skin with ...

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Thank you for contacting us.

We have several antibodies against beta galactosidase, two of which have been validated for IHC staining of paraffin embedded sections. You may want to consider chicken polyclonal ab9361 or rabbit polyclonal ab47...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"