Anti-beta Galactosidase抗体[2E9] (ab10074)

概述

  • 产品名称Anti-beta Galactosidase抗体[2E9]
    参阅全部 beta Galactosidase 一抗
  • 描述
    小鼠单克隆抗体[2E9] to beta Galactosidase
  • 经测试应用适用于: ELISAmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Full length protein (expressed in E. coli).

  • 常规说明This antibody does not influence enzyme activity. Concentration varies from lot to lot and can be provided on request.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purity tested by electrophoresis.
  • 克隆单克隆
  • 克隆编号2E9
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
  • 应用说明IA: Detection of b-galactosidase expressed by LacZ gene in cloned sequences.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • 细胞定位Cytoplasmic
    • 数据库链接
      • 别名
        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      Anti-beta Galactosidase antibody [2E9] (ab10074)参考文献

      ab10074 has not yet been referenced specifically in any publications.

      Product Wall

      Thank you for your reply. I have checked the sequence homology between beta-galactosidase of E.coli (P00722) with Aspergillus oryzae (CAW30743.1). The alignment is very poor and shows only 22-33% homology for short regions, otherwise the homolo...

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      Thank you for contacting us. I am checking with the lab regarding ab92417 and if cross reactivity with alpha-galactosidase has been tested, and will let you know as soon as I hear back. For both antibodies (ab92417, ab10074): Both were o...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"