Anti-beta Dystroglycan (phospho Y892)抗体[M117] (ab29037)

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概述

  • 产品名称
    Anti-beta Dystroglycan (phospho Y892)抗体[M117]
    参阅全部 beta Dystroglycan 一抗
  • 描述
    小鼠单克隆抗体[M117] to beta Dystroglycan (phospho Y892)
  • 特异性
    Ab29037 recognises beta Dystroglycan (phospho Y892).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 892 of human dystroglycan.

  • 阳性对照
    • HepG2 cells or HeLa cells treated with pervanadate.

性能

应用

Our Abpromise guarantee covers the use of ab29037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: 1/2000.
    WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 相关性
      Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
    • 细胞定位
      Type I membrane protein.
    • 数据库链接
      • 别名
        • 156DAG antibody
        • A3a antibody
        • AGRNR antibody
        • Beta DG antibody
        • Beta dystroglycan antibody
        • DAG 1 antibody
        • DAG1 antibody
        • Dystroglycan antibody
        • Dystrophin associated glycoprotein 1 antibody
        see all

      Anti-beta Dystroglycan (phospho Y892) antibody [M117] 图像

      • Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

        Lane 1 : Lysates from HepG2 cells (untreated)
        Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min

        Lysates/proteins at 20 µg per lane.


        Predicted band size : 97 kDa
        Observed band size : 43 kDa (why is the actual band size different from the predicted?)
      • Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)


        Predicted band size : 97 kDa

      实验方案

      Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)参考文献

      ab29037 has not yet been referenced specifically in any publications.

      发表研究结果有使用 ab29037？请让我们知道，以便我们可以引用本数据表中的参考文章。

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      I am currently investigating the antibodies against dystroglycan both alpha and beta and have seen that you have a large selection of different clones available. The antibodies with product codes mentioned above state they have not been referenced in p...

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      Thank you for your inquiry.

      I can confirm that we do not know of any publication using the above mentioned antibodies. I would like to reassure you that we do guarantee our product for all species and applications stated on the respective dat...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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