概述
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产品名称Anti-beta Dystroglycan (phospho Y892)抗体[M117]
参阅全部 beta Dystroglycan 一抗 -
描述小鼠单克隆抗体[M117] to beta Dystroglycan (phospho Y892)
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宿主Mouse
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特异性Ab29037 recognises beta Dystroglycan (phospho Y892).
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 892 of human dystroglycan.
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阳性对照
- HepG2 cells or HeLa cells treated with pervanadate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.05% Sodium Azide.
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号M117
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab29037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
应用说明
ELISA: 1/2000.
WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
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细胞定位Type I membrane protein.
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数据库链接
- Entrez Gene: 1605 Human
- Entrez Gene: 13138 Mouse
- Entrez Gene: 114489 Rat
- Omim: 128239 Human
- SwissProt: Q14118 Human
- SwissProt: Q62165 Mouse
- Unigene: 76111 Human
- Unigene: 36260 Rat
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别名
- 156DAG antibody
- A3a antibody
- AGRNR antibody
see all
图片
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![Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)](http://www.abcam.cn/ps/datasheet/Images/29/ab29037/ab29037_2.jpg)
Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
Lane 1 : Lysates from HepG2 cells (untreated)
Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Observed band size: 43 kDa (why is the actual band size different from the predicted?)
Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa. -
![Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)](http://www.abcam.cn/ps/datasheet/Images/29/ab29037/ab29037_3.jpg)
Western blot - beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).
文献
ab29037 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"