Anti-beta Dystroglycan抗体(ab43125)

概述

  • 产品名称Anti-beta Dystroglycan抗体
    参阅全部 beta Dystroglycan 一抗
  • 描述
    兔多克隆抗体to beta Dystroglycan
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Dystroglycan precursor.

    (Peptide available as ab43750.)

  • 阳性对照
    • ab43125 gave a positive signal in the following human tissue lysates: Brain; Heart; Skeletal Muscle; Testes (data not shown); Kidney (data not shown); Colon (data not shown)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab43125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

靶标

  • 相关性Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
  • 细胞定位Type I membrane protein.
  • 数据库链接
  • 别名
    • 156DAG antibody
    • A3a antibody
    • AGRNR antibody
    • Beta DG antibody
    • Beta dystroglycan antibody
    • DAG 1 antibody
    • DAG1 antibody
    • Dystroglycan antibody
    • Dystrophin associated glycoprotein 1 antibody
    see all

Anti-beta Dystroglycan antibody 图像

  • All lanes : Anti-beta Dystroglycan antibody (ab43125) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

Anti-beta Dystroglycan antibody (ab43125)参考文献

ab43125 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (muscle)
Permeabilization No
Specification muscle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative no fixative
Username

Miss. Laurence Richard

Verified customer

提交于 Sep 23 2015

Have you tried increasing the concentration of ab64568? It does look like the MAb is reacting at the correct area but there does appear to be some issue which is affecting the antibody/antigen binding and preventing good visualization. Have you attempt...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Oct 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"