Anti-beta Dystroglycan抗体(ab43125)
Key features and details
- Rabbit polyclonal to beta Dystroglycan
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-beta Dystroglycan抗体
参阅全部 beta Dystroglycan 一抗 -
描述
兔多克隆抗体to beta Dystroglycan -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Cow, Dog -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Dystroglycan precursor.
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阳性对照
- WB: Human brain, heart, skeletal muscle, testes (data not shown), kidney (data not shown), colon (data not shown) tissue lysates and HEK293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab43125于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
靶标
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相关性
Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation. -
细胞定位
Type I membrane protein. -
数据库链接
- Entrez Gene: 281439 Cow
- Entrez Gene: 476623 Dog
- Entrez Gene: 1605 Human
- Entrez Gene: 13138 Mouse
- Entrez Gene: 100009278 Rabbit
- Omim: 128239 Human
- SwissProt: O18738 Cow
- SwissProt: Q9TSZ6 Dog
see all -
别名
- 156DAG antibody
- A3a antibody
- AGRNR antibody
see all
图片
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All lanes : Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAG1 knockout HEK293T cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDaLanes 1-3: Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-beta Dystroglycan antibody (ab43125) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab43125 被引用在 5 文献中.
- Murphy S et al. Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle. J Proteomics 191:212-227 (2019). PubMed: 29408692
- Trajanovska S et al. Muscle specific kinase protects dystrophic mdx mouse muscles from eccentric contraction-induced loss of force-producing capacity. J Physiol 597:4831-4850 (2019). PubMed: 31340406
- Murphy S et al. Chemical crosslinking analysis of β-dystroglycan in dystrophin-deficient skeletal muscle. HRB Open Res 1:17 (2018). PubMed: 35528858
- Awad PN et al. KCC2 Regulates Dendritic Spine Formation in a Brain-Region Specific and BDNF Dependent Manner. Cereb Cortex 28:4049-4062 (2018). PubMed: 30169756
- Sciandra F et al. Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene. BMC Res Notes 10:601 (2017). PubMed: 29157305