Anti-beta Defensin 3抗体(ab19270)


  • 产品名称Anti-beta Defensin 3抗体
    参阅全部 beta Defensin 3 一抗
  • 描述
    兔多克隆抗体to beta Defensin 3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant full length human beta defensin 3.



Our Abpromise guarantee covers the use of ab19270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23627872


  • 功能Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
  • 组织特异性Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
  • 序列相似性Belongs to the beta-defensin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BD 3 antibody
    • BD-3 antibody
    • BD3 antibody
    • beta 103 antibody
    • Beta defensin 103A antibody
    • Beta defensin 103A precursor antibody
    • Beta defensin 3 antibody
    • Beta-defensin 103 antibody
    • Beta-defensin 3 antibody
    • D103A_HUMAN antibody
    • DEF B3 antibody
    • DEFB 103 antibody
    • DEFB 103A antibody
    • DEFB 3 antibody
    • DEFB-3 antibody
    • DEFB103 antibody
    • DEFB103A antibody
    • DEFB103B antibody
    • DEFB3 antibody
    • Defensin antibody
    • Defensin beta 103A antibody
    • Defensin beta 3 antibody
    • Defensin like protein antibody
    • Defensin-like protein antibody
    • HBD 3 antibody
    • hBD-3 antibody
    • HBD3 antibody
    • HBP 3 antibody
    • HBP3 antibody
    • mBD3 antibody
    see all

Anti-beta Defensin 3 antibody 图像

  • ab19270 staining beta Defensin 3 in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An  Alexa Fluor®488-conjugated  was used as the secondary antibody and DAPI used to stain the nuclei.

Anti-beta Defensin 3 antibody (ab19270)参考文献

This product has been referenced in:
  • Pierson T  et al. Cigarette smoke extract induces differential expression levels of beta-defensin peptides in human alveolar epithelial cells. Tob Induc Dis 11:10 (2013). ICC/IF ; Human . Read more (PubMed: 23627872) »
  • Maisetta G  et al. Evaluation of the inhibitory effects of human serum components on bactericidal activity of human beta defensin 3. Peptides 29:1-6 (2008). Read more (PubMed: 18045738) »

See all 2 Publications for this product

Product Wall

Sure, I am happy to send along the replacements.

If you could please remind me of the purchase order number or Abcam order reference number associated with the original purchase of these antibodies, I'll arrange replacements immediately.
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Application Immunohistochemistry (Frozen sections)
Sample Equus Tissue sections (respiratory tract)
Permeabilization Yes - 0.1% Triton X 100
Specification respiratory tract
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde

Jolien Van Cleemput

Verified customer

提交于 Dec 07 2015

Does your customer want to use the same catalog numbers or would she be interested in choosing one or more different antibodies (of the same price) to the beta defensin proteins?

Thanks for passing along the peptide and protein concentration information.

These concentrations seem reasonable.

Is your customer interested in replacements for the antibodies?

Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

Here are my thoughts:

1. In general it looks like the antibodies are able to detect Defensin in the chorioam...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubl...

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Thank you for your enquiry. The immunogen was recombinant human beta-defensin-3. This same material was used in Western blots using an SDS-PAGE mini gel. I have updated the datasheet to reflect that the immunogen is recombinant. Please let us k...

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