Anti-beta COP抗体(ab24359)

概述

  • 产品名称Anti-beta COP抗体
    参阅全部 beta COP 一抗
  • 描述
    兔多克隆抗体to beta COP
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Rat beta COP.

  • 阳性对照
    • Rat and mouse brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab24359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity). Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
  • 序列相似性Contains 6 HEAT repeats.
  • 翻译后修饰Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8.
  • 细胞定位Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. Cell membrane. Endoplasmic reticulum-Golgi intermediate compartment. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta-coat protein antibody
    • Beta-COP antibody
    • betacop antibody
    • Coatomer beta subunit antibody
    • Coatomer protein complex subunit beta 1 antibody
    • Coatomer protein complex subunit beta antibody
    • Coatomer subunit beta antibody
    • COPB antibody
    • COPB_HUMAN antibody
    • Copb1 antibody
    • DKFZp761K102 antibody
    • FLJ10341 antibody
    see all

Anti-beta COP antibody 图像

  • All lanes : Anti-beta COP antibody (ab24359) at 1 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    See Abreview

  • Image courtesy of Human Protein Atlas

    ab24359 staining beta COP in paraffin embedded Human pancreas. The tissue was incubated with ab24359 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab24359 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab24359, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-beta COP antibody (ab24359)参考文献

ab24359 has not yet been referenced specifically in any publications.

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DISCOUNT CODE: XXXXXXXXX (ab6323)
DISCOUNT CODE: XXXXXXXXX (ab24359)
Expiration date: 30-11-2012
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